Catalog No: OKCD01859 (Formerly GWB-KBBAK4)
Size:96WELLS
Price: $700.00
SKU
OKCD01859
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Datasheets/ManualsPrintable datasheet for Cacna1e ELISA Kit (Mouse) (OKCD01859)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cacna1e Microplate96 Wells (12 x 8 Well Strips)
Cacna1e Lyophilized Standard2 x 20 ng
100X Biotinylated Cacna1e Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.125 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E). No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCACNA1E
Gene Full NameCalcium channel, voltage-dependent, R type, alpha 1E subunit
Alias SymbolsBrain calcium channel II;Calcium channel, L type, alpha-1 polypeptide, isoform 6;Voltage-gated calcium channel subunit alpha Cav2.3.
NCBI Gene Id12290
Protein NameVoltage-dependent R-type calcium channel subunit alpha-1E
Description of TargetVoltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents. R-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by nickel, and partially by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), omega-conotoxin-GVIA (omega-CTx-GVIA), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1E subunit could be involved in the modulation of firing patterns of neurons which is important for information processing.
Uniprot IDQ61290
Protein Accession #NP_033912.2
Nucleotide Accession #NM_009782.3
Protein Size (# AA)2272
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