CYSLTR1 ELISA Kit (Human) (OKCD01674)

Catalog No: OKCD01674 (Formerly GWB-KBBJS2)

Size:96WELLS

Human CYSLTR1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteinyl Leukotriene Receptor 1 (CYSLTR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteinyl Leukotriene Receptor 1 (CYSLTR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteinyl Leukotriene Receptor 1 (CYSLTR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteinyl Leukotriene Receptor 1 (CYSLTR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteinyl Leukotriene Receptor 1 (CYSLTR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteinyl Leukotriene Receptor 1 (CYSLTR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CYSLTR1 Microplate: 96 Wells (12 x 8 Well Strips)
  • CYSLTR1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CYSLTR1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.135 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cysteinyl Leukotriene Receptor 1 (CYSLTR1). No significant cross-reactivity or interference between Cysteinyl Leukotriene Receptor 1 (CYSLTR1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CYSLTR1
• Gene Full Name: Cysteinyl leukotriene receptor 1
• Alias Symbols: CYSLT1, CYSLTR, CYSLT1R, HMTMF81
• NCBI Gene Id: 10800
• Protein Name: Cysteinyl leukotriene receptor 1
• Description of Target: Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.
• Uniprot ID: Q9Y271
• Protein Accession #: NP_001269115.1
• Nucleotide Accession #: NM_001282186.1
• Protein Size (# AA): 337