CYP19A1 ELISA Kit (Rat) (OKCA00967)

Catalog No: OKCA00967

Size:96 Wells

Rat CYP19A1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	101	95-107
  1:2	91	86-97
  1:4	88	84-92
  1:8	95	90-100

• ELISA Kit Principle: Aviva Systems Biology CYP19A1 ELISA Kit (Rat) (OKCA00967) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CYP19A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP19A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CYP19A1 captured in well.
• ELISA Kit Range: 18.75 pg/mL-1,200 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85	80-90
  EDTA Plasma (n=4)	96	91-103

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level CYP19A1 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level CYP19A1 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • CYP19A1 Microplate: 96 Wells (12 x 8 Well strips)
  • CYP19A1 Lyophilized Standard: 2
  • 100X Biotinylated CYP19A1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 4.68 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CYP19A1
• Gene Full Name: Aromatase
• NCBI Gene Id: 100328545
• Protein Name: Aromatase
• Description of Target: Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
• Uniprot ID: Q29605
• Protein Accession #: NP_001164392.1
• Nucleotide Accession #: NM_001170921.2