COL1A2 ELISA Kit (Rabbit) (OKCD01499)

Catalog No: OKCD01499 (Formerly GWB-KBBJL9)

Size:96WELLS

Rabbit COL1A2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Oryctolagus cuniculus|Rabbit
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	87-102%	96-104%	86-104%	93-101%
  EDTA Plasma (n=5)	92-99%	82-97%	79-93%	89-105%
  Heparin Plasma (n=5)	89-103%	80-95%	96-105%	91-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type I Alpha 2 (COL1a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	78-95	92
  EDTA Plasma (n=5)	78-95	82
  Heparin Plasma (n=5)	89-99	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-COL1A2 Microplate: 96 Wells (12 x 8 Well Strips)
  • COL1A2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated COL1A2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.112 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 2 (COL1a2). No significant cross-reactivity or interference between Collagen Type I Alpha 2 (COL1a2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: COL1A2
• Alias Symbols: Alpha-2 type I collagen.
• NCBI Gene Id: 100008997
• Protein Name: Collagen alpha-2(I) chain
• Description of Target: Type I collagen is a member of group I collagen (fibrillar forming collagen).
• Uniprot ID: Q28668
• Protein Accession #: NP_001182597.1
• Nucleotide Accession #: NM_001195668.1
• Protein Size (# AA): 526