COL11A2 ELISA Kit (Human) (OKCD01636)

Catalog No: OKCD01636 (Formerly GWB-KBBIB8)

Size:96WELLS

Human COL11A2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	88-103%	84-101%	90-99%	79-95%
  EDTA Plasma (n=5)	78-101%	97-104%	93-102%	80-105%
  Heparin Plasma (n=5)	88-96%	78-97%	79-90%	98-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type XI Alpha 2 (COL11a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type XI Alpha 2 (COL11a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type XI Alpha 2 (COL11a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type XI Alpha 2 (COL11a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	78-91	87
  EDTA Plasma (n=5)	97-104	101
  Heparin Plasma (n=5)	82-101	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type XI Alpha 2 (COL11a2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type XI Alpha 2 (COL11a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-COL11A2 Microplate: 96 Wells (12 x 8 Well Strips)
  • COL11A2 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated COL11A2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.067 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type XI Alpha 2 (COL11a2). No significant cross-reactivity or interference between Collagen Type XI Alpha 2 (COL11a2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: COL11A2
• Gene Full Name: Collagen type XI alpha 2 chain
• Alias Symbols: HKE5, PARP, STL3, FBCG2, DFNA13, DFNB53, OSMEDA, OSMEDB
• NCBI Gene Id: 1302
• Protein Name: Collagen alpha-2(XI) chain
• Description of Target: May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
• Uniprot ID: P13942
• Protein Accession #: NP_001157243.1
• Nucleotide Accession #: NM_001163771.1
• Protein Size (# AA): 1736