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Catalog No: OKCD02043 (Formerly GWB-KBBAD3)
Size:1 unit
Price: $75.00
SKU
OKCD02043
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3.5 Hours
ELISA Kit PrincipleAviva Systems Biology CLEC7A ELISA Kit (Human) (OKCD02043) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLEC7A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLEC7A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CLEC7A captured in the well.
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CLEC7A was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CLEC7A was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-CLEC7A Microplate96 Wells (12 x 8 Well Strips)
CLEC7A Lyophilized Standard2 x 10 ng
100X Biotinylated CLEC7A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.064 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of C-Type Lectin Domain Family 7, Member A (CLEC7A). No significant cross-reactivity or interference between C-Type Lectin Domain Family 7, Member A (CLEC7A) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCLEC7A
Gene Full NameC-type lectin domain family 7 member A
Alias SymbolsBGR, CD369, CANDF4, SCARE2, DECTIN1, CLECSF12
NCBI Gene Id64581
Protein NameC-type lectin domain family 7 member A
Description of TargetLectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
Uniprot IDQ9BXN2
Protein Accession #NP_072092.2
Nucleotide Accession #NM_022570.4
Protein Size (# AA)247
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