Catalog No: OKCD02043 (Formerly GWB-KBBAD3)
Size:1 unit
Price: $75.00
SKU
OKCD02043
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3.5 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology CLEC7A ELISA Kit (Human) (OKCD02043) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLEC7A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLEC7A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CLEC7A captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156 - 10 ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with known low, middle and high level CLEC7A was tested with 20 replicates across one plate. Inter-assay Precision: 3 samples with known low, middle and high level CLEC7A was tested on three different plates with 8 replicates, respectively. Intra-Assay CV: <10% Inter-Assay CV: <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.064 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of C-Type Lectin Domain Family 7, Member A (CLEC7A). No significant cross-reactivity or interference between C-Type Lectin Domain Family 7, Member A (CLEC7A) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | CLEC7A |
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Gene Full Name | C-type lectin domain family 7 member A |
Alias Symbols | BGR, CD369, CANDF4, SCARE2, DECTIN1, CLECSF12 |
NCBI Gene Id | 64581 |
Protein Name | C-type lectin domain family 7 member A |
Description of Target | Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. |
Uniprot ID | Q9BXN2 |
Protein Accession # | NP_072092.2 |
Nucleotide Accession # | NM_022570.4 |
Protein Size (# AA) | 247 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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