Catalog No: OKCD01999 (Formerly GWB-KBBYY9)
Size:96WELLS
Price: $675.00
SKU
OKCD01999
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for CHST7 ELISA Kit (Human) (OKCD01999) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Sulfotransferase 7 (CHST7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbohydrate Sulfotransferase 7 (CHST7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbohydrate Sulfotransferase 7 (CHST7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbohydrate Sulfotransferase 7 (CHST7) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 78-5,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbohydrate Sulfotransferase 7 (CHST7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbohydrate Sulfotransferase 7 (CHST7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 31 pg/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Carbohydrate Sulfotransferase 7 (CHST7). No significant cross-reactivity or interference between Carbohydrate Sulfotransferase 7 (CHST7) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | CHST7 |
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Gene Full Name | carbohydrate sulfotransferase 7 |
Alias Symbols | C6ST-2;carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7;carbohydrate sulfotransferase 7;Chondroitin 6-sulfotransferase 2;chondroitin 6-sulfotransferase-2;galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5;glcNAc6ST-4;gn6st-4;GST-5;N-acetylglucosamine 6-O-sulfotransferase 4. |
NCBI Gene Id | 56548 |
Protein Name | Carbohydrate sulfotransferase 7 |
Description of Target | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc. |
Uniprot ID | Q9NS84 |
Protein Accession # | NP_063939.2 |
Nucleotide Accession # | NM_019886.3 |
Protein Size (# AA) | 486 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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