CEMP1 ELISA Kit (Human) (OKCD02075)

Catalog No: OKCD02075 (Formerly GWB-KBBKW3)

Size:96WELLS

Human CEMP1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cementum Protein 1 (CEMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cementum Protein 1 (CEMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cementum Protein 1 (CEMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cementum Protein 1 (CEMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cementum Protein 1 (CEMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cementum Protein 1 (CEMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CEMP1 Microplate: 96 Wells (12 x 8 Well Strips)
  • CEMP1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CEMP1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.122 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cementum Protein 1 (CEMP1). No significant cross-reactivity or interference between Cementum Protein 1 (CEMP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CEMP1
• Gene Full Name: cementum protein 1
• Alias Symbols: cementoblastoma-derived protein 1;Cementum protein 1;cementum protein 23;CP23;CP-23.
• NCBI Gene Id: 752014
• Protein Name: Cementoblastoma-derived protein 1
• Description of Target: May play a role in development of the periodontium which surrounds and supports the teeth by promoting the differentiation of multi-potent cells from the periodontal ligament into cementoblasts to form the cementum (PubMed:21929512, PubMed:17509525, PubMed:21465469). Binds hydroxyapatite and may promote the biomineralization of the cementum (PubMed:19393626). Also promotes cell proliferation (PubMed:17509525, PubMed:21929512, PubMed:26011628).
• Uniprot ID: Q6PRD7
• Protein Accession #: NP_001041677.1
• Nucleotide Accession #: NM_001048212.3
• Protein Size (# AA): 247