CD99 ELISA Kit (Human) (OKCD01585)

Catalog No: OKCD01585 (Formerly GWB-KBBKM4)

Size:96WELLS

Human CD99 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	89-96%	82-97%	78-94%	95-105%
  EDTA Plasma (n=5)	83-101%	89-97%	99-105%	78-105%
  Heparin Plasma (n=5)	97-105%	85-101%	78-98%	92-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 99 (CD99). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 99 (CD99). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 99 (CD99), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 99 (CD99) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	82-105	86
  EDTA Plasma (n=5)	80-91	87
  Heparin Plasma (n=5)	80-103	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 99 (CD99) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 99 (CD99) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CD99 Microplate: 96 Wells (12 x 8 Well Strips)
  • CD99 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CD99 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.065 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 99 (CD99). No significant cross-reactivity or interference between Cluster Of Differentiation 99 (CD99) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CD99
• Gene Full Name: CD99 molecule (Xg blood group)
• Alias Symbols: 12E7;antigen identified by monoclonal 12E7, Y homolog;antigen identified by monoclonal antibodies 12E7, F21 and O13;CD99 antigen;cell surface antigen 12E7;cell surface antigen HBA-71;cell surface antigen O13;E2 antigen;HBA71;MIC2;MIC2 (monoclonal antibody 12E7);MIC2X;MIC2Y;MSK5X;Protein MIC2;surface antigen MIC2;T-cell surface glycoprotein E2.
• NCBI Gene Id: 4267
• Protein Name: CD99 antigen
• Description of Target: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
• Uniprot ID: P14209
• Protein Accession #: NP_001116370.1
• Nucleotide Accession #: NM_001122898.2
• Protein Size (# AA): 185