CD247 ELISA Kit (Human) (OKCA00832)

Catalog No: OKCA00832

Size:96 Wells

Human CD247 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	89	83-94
  1:2	95	90-99
  1:4	106	100-115
  1:8	84	80-88

• ELISA Kit Principle: Aviva Systems Biology CD247 ELISA Kit (Human) (OKCA00832) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CD247 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD247 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CD247 captured in well.
• ELISA Kit Range: 0.156 ng/mL-10 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	98	93-102
  EDTA Plasma (n=4)	93	89-97

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level CD247 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level CD247 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • CD247 Microplate: 96 Wells (12 x 8 Well strips)
  • CD247 Lyophilized Standard: 2
  • 100X Biotinylated CD247 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 0.039 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CD247
• Gene Full Name: CD247 molecule
• Alias Symbols: T3Z, CD3H, CD3Q, CD3Z, TCRZ, IMD25, CD3-ZETA
• NCBI Gene Id: 919
• Protein Name: T-cell surface glycoprotein CD3 zeta chain
• Description of Target: Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
• Uniprot ID: P20963
• Protein Accession #: NP_000725.1
• Nucleotide Accession #: NM_000734.3