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Catalog No: OKCD02079 (Formerly GWB-KBBEQ2)
Size:96WELLS
Price: $750.00
SKU
OKCD02079
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Datasheets/ManualsPrintable datasheet for CD200R1 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)84-96%80-99%78-97%85-99%
EDTA Plasma (n=5)92-101%97-105%86-97%78-96%
Heparin Plasma (n=5)93-101%93-101%89-101%84-99%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD200 Receptor 1 (CD200R1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CD200 Receptor 1 (CD200R1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD200 Receptor 1 (CD200R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CD200 Receptor 1 (CD200R1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.62-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)83-9087
EDTA Plasma (n=5)79-10190
Heparin Plasma (n=5)87-10496
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CD200 Receptor 1 (CD200R1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CD200 Receptor 1 (CD200R1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CD200R1 Microplate96 Wells (12 x 8 Well Strips)
CD200R1 Lyophilized Standard2 x 1 ng
100X Biotinylated CD200R1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 6.6 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of CD200 Receptor 1 (CD200R1). No significant cross-reactivity or interference between CD200 Receptor 1 (CD200R1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCD200R1
Gene Full NameCD200 receptor 1
Alias SymbolsOX2R, MOX2R, CD200R, HCRTR2
NCBI Gene Id131450
Protein NameCell surface glycoprotein CD200 receptor 1
Description of TargetInhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
Uniprot IDQ8TD46
Protein Accession #NP_620161.1
Nucleotide Accession #NM_138806.3
Protein Size (# AA)325
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