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Catalog No: OKCA00626
Size:96 Wells
Price: $754.00
SKU
OKCA00626
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9186-95
1:210297-107
1:49185-94
1:89591-99
ELISA Kit PrincipleAviva Systems Biology C5AR1 ELISA Kit (Human) (OKCA00626) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C5AR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C5AR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C5AR1 captured in the well.
ELISA Kit Range31.25 pg/mL-2,000 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9589-98
EDTA Plasma (n=4)9288-96
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level C5AR1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level C5AR1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
C5AR1 Microplate96 Wells (12 x 8 Well strips)
C5AR1 Lyophilized Standard2
100X Biotinylated C5AR1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity7.8 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolC5AR1
Gene Full NameComplement C5a receptor 1
Alias SymbolsC5A, C5AR, C5R1, CD88
NCBI Gene Id728
Protein NameC5a anaphylatoxin chemotactic receptor 1
Description of TargetReceptor for the chemotactic and inflammatory peptide anaphylatoxin C5a . The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events . Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production .
Uniprot IDP21730
Protein Accession #NP_001727.1
Nucleotide Accession #NM_001736.3
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