BMP8A ELISA Kit (Human) (OKCD01625)

Catalog No: OKCD01625 (Formerly GWB-KBBOS2)

Size:96WELLS

Human BMP8A ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	87-95%	94-103%	93-105%	98-105%
  EDTA Plasma (n=5)	80-94%	93-102%	86-104%	93-104%
  Heparin Plasma (n=5)	88-101%	78-101%	78-89%	82-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 8A (BMP8A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 8A (BMP8A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 8A (BMP8A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein 8A (BMP8A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	93-105	101
  EDTA Plasma (n=5)	85-92	89
  Heparin Plasma (n=5)	88-97	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 8A (BMP8A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 8A (BMP8A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BMP8A Microplate: 96 Wells (12 x 8 Well Strips)
  • BMP8A Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated BMP8A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 6.0 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 8A (BMP8A). No significant cross-reactivity or interference between Bone Morphogenetic Protein 8A (BMP8A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: BMP8A
• Gene Full Name: bone morphogenetic protein 8a
• Alias Symbols: BMP-8A;bone morphogenetic protein 8A;OP-2.
• NCBI Gene Id: 353500
• Protein Name: Bone morphogenetic protein 8A
• Description of Target: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis (By similarity). Signaling protein involved in regulation of thermogenesis and energy balance. Proposed to increase the peripheral response of brown adipose tissue (BAT) to adrenergic stimulation while acting centrally in the hypothalamus to increase sympathetic output to BAT.
• Uniprot ID: Q7Z5Y6
• Protein Accession #: NP_861525.2
• Nucleotide Accession #: NM_181809.3
• Protein Size (# AA): 402