Catalog No: OKCD02006 (Formerly GWB-KBBXA4)
Size:96WELLS
Price: $675.00
SKU
OKCD02006
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Datasheets/ManualsPrintable datasheet for BFAR ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)92-101%78-104%94-101%86-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bifunctional Apoptosis Regulator (BFAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bifunctional Apoptosis Regulator (BFAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bifunctional Apoptosis Regulator (BFAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bifunctional Apoptosis Regulator (BFAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum97-104101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bifunctional Apoptosis Regulator (BFAR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bifunctional Apoptosis Regulator (BFAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-BFAR Microplate96 Wells (12 x 8 Well Strips)
BFAR Lyophilized Standard2 x 10 ng
100X Biotinylated BFAR Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Bifunctional Apoptosis Regulator (BFAR). No significant cross-reactivity or interference between Bifunctional Apoptosis Regulator (BFAR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolBFAR
Gene Full NameBifunctional apoptosis regulator
Alias SymbolsBAR, RNF47
NCBI Gene Id51283
Protein NameBifunctional apoptosis regulator
Description of TargetApoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors.
Uniprot IDQ9NZS9
Protein Accession #NP_057645.1
Nucleotide Accession #NM_016561.2
Protein Size (# AA)450
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