Catalog No: OKCD02040 (Formerly GWB-KBBMZ5)
Size:96WELLS
Price: $750.00
SKU
OKCD02040
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Datasheets/ManualsPrintable datasheet for BCL2A1 ELISA kit (Human)
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)96-104%88-95%98-105%97-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Related Protein A1 (BCL2A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Related Protein A1 (BCL2A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Related Protein A1 (BCL2A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bcl2 Related Protein A1 (BCL2A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum91-9894
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Related Protein A1 (BCL2A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Related Protein A1 (BCL2A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-BCL2A1 Microplate96 Wells (12 x 8 Well Strips)
BCL2A1 Lyophilized Standard2 x 10 ng
100X Biotinylated BCL2A1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.065 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Bcl2 Related Protein A1 (BCL2A1). No significant cross-reactivity or interference between Bcl2 Related Protein A1 (BCL2A1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolBCL2A1
Gene Full NameBCL2 related protein A1
Alias SymbolsACC1;ACC-1;ACC2;ACC-2;bcl2-L-5;BCL2L5;bcl-2-like protein 5;bcl-2-related protein A1;BFL1;GRS;HBPA1;hematopoietic BCL2-related protein A1;hemopoietic-specific early response protein;protein BFL-1;Protein GRS.
NCBI Gene Id597
Protein NameQ16548
Description of TargetBcl-2-related protein A1
Uniprot IDhttps://www.uniprot.org/uniprot/Q16548
Protein Accession #NP_001108207.1
Nucleotide Accession #NM_001114735.1
Protein Size (# AA)175
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