BCAN ELISA Kit (Human) (OKCD01645)

Catalog No: OKCD01645 (Formerly GWB-KBBLJ7)

Size:96WELLS

Human BCAN ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brevican (BCAN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brevican (BCAN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brevican (BCAN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Brevican (BCAN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brevican (BCAN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brevican (BCAN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BCAN Microplate: 96 Wells (12 x 8 Well Strips)
  • BCAN Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated BCAN Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.128 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Brevican (BCAN). No significant cross-reactivity or interference between Brevican (BCAN) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: BCAN
• Gene Full Name: brevican
• Alias Symbols: BEHAB;brain-enriched hyaluronan-binding protein;brevican core protein;chondroitin sulfate proteoglycan 7;chondroitin sulfate proteoglycan BEHAB;CSPG7.
• NCBI Gene Id: 63827
• Protein Name: Brevican core protein
• Description of Target: May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.
• Uniprot ID: Q96GW7
• Protein Accession #: NP_068767.3
• Nucleotide Accession #: NM_021948.4
• Protein Size (# AA): 911