BASP1 ELISA Kit (Human) (OKCD02007)

Catalog No: OKCD02007 (Formerly GWB-KBBAF4)

Size:96WELLS

Human BASP1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Abundant, Membrane Attached Signal Protein 1 (BASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Abundant, Membrane Attached Signal Protein 1 (BASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Abundant, Membrane Attached Signal Protein 1 (BASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CILP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • CILP2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CILP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.114 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Brain Abundant, Membrane Attached Signal Protein 1 (BASP1). No significant cross-reactivity or interference between Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) and analogues was observed.
• Publications:

  A novel urinary biomarker protein panel to identify children with ureteropelvic junction obstruction - A pilot study. J Pediatr Urol. 16, 466.e1-466.e9 (2020). 32620509

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for BASP1 ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: BASP1
• Gene Full Name: brain abundant membrane attached signal protein 1
• Alias Symbols: 22 kDa neuronal tissue-enriched acidic protein;brain acid soluble protein 1;brain acid-soluble protein 1;CAP23;CAP-23;NAP22;NAP-22;neuronal axonal membrane protein NAP-22;neuronal tissue-enriched acidic protein.
• NCBI Gene Id: 10409
• Protein Name: Brain acid soluble protein 1
• Description of Target: Brain acid soluble protein 1
• Uniprot ID: P80723
• Protein Accession #: NP_001258535.1
• Nucleotide Accession #: NM_001271606.1
• Protein Size (# AA): 227