Catalog No: OKCD02095 (Formerly GWB-KBBDK5)
Size:96WELLS
Price: $750.00
SKU
OKCD02095
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Datasheets/ManualsPrintable datasheet for ATP1b4 ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Atp1b4 Microplate96 Wells (12 x 8 Well Strips)
Atp1b4 Lyophilized Standard2 x 10 ng
100X Biotinylated Atp1b4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolATP1B4
Gene Full NameATPase, (Na+)/K+ transporting, beta 4 polypeptide
Alias Symbols1110020B02Rik
NCBI Gene Id67821
Protein NameProtein ATP1B4
Description of TargetMay act as a transcriptional coregulator during muscle development through its interaction with SNW1. Has lost its ancestral function as a Na,K-ATPase beta-subunit.
Uniprot IDQ99ME6
Protein Accession #NP_001277318.1
Nucleotide Accession #NM_001290389.1
Protein Size (# AA)356
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