ATP1B4 ELISA Kit (Human) (OKCD02094)

Catalog No: OKCD02094 (Formerly GWB-KBBMV6)

Size:96WELLS

Human ATP1B4 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ATP1B4 Microplate: 96 Wells (12 x 8 Well Strips)
  • ATP1B4 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ATP1B4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.106 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ATP1B4
• Gene Full Name: ATPase Na+/K+ transporting family member beta 4
• Alias Symbols: Protein ATP1B4, X,K-ATPase subunit beta-m, X/potassium-transporting ATPase subunit beta-m
• NCBI Gene Id: 23439
• Protein Name: Protein ATP1B4
• Description of Target: May act as a transcriptional coregulator during muscle development through its interaction with SNW1. Has lost its ancestral function as a Na,K-ATPase beta-subunit.
• Uniprot ID: Q9UN42
• Protein Accession #: NP_001135919.1
• Nucleotide Accession #: NM_001142447.2
• Protein Size (# AA): 357