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Catalog No: OKCA00620
Size:96 Wells
Price: $857.00
SKU
OKCA00620
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
1:510096-104
1:108683-89
1:208985-94
1:40103100-106
ELISA Kit PrincipleAviva Systems Biology APOC1 ELISA Kit (Human) (OKCA00620) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APOC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for APOC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample APOC1 captured in the well.
ELISA Kit Range0.312 ug/mL-20 ug/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9993-104
EDTA Plasma (n=4)8783-90
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level APOC1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level APOC1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
APOC1 Microplate96 Wells (12 x 8 Well strips)
APOC1 Lyophilized Standard2
100X Biotinylated APOC1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma
Sensitivity0.078 ug/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolAPOC1
Gene Full NameApolipoprotein C-I
Protein NameApolipoprotein C-I
Description of TargetInhibitor of lipoprotein binding to the low density lipoprotein (LDL) receptor, LDL receptor-related protein, and very low density lipoprotein (VLDL) receptor. Associates with high density lipoproteins (HDL) and the triacylglycerol-rich lipoproteins in the plasma and makes up about 10% of the protein of the VLDL and 2% of that of HDL. Appears to interfere directly with fatty acid uptake and is also the major plasma inhibitor of cholesteryl ester transfer protein (CETP). Binds free fatty acids and reduces their intracellular esterification. Modulates the interaction of APOE with beta-migrating VLDL and inhibits binding of beta-VLDL to the LDL receptor-related protein.
Uniprot IDP33047
Protein Accession #NP_001153753.1
Nucleotide Accession #NM_001160281.2
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