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Catalog No: OKCD01846 (Formerly GWB-KBBYM2)
Size:96WELLS
Price: $675.00
SKU
OKCD01846
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Datasheets/ManualsPrintable datasheet for ADH6 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)96-104%96-103%87-94%93-101%
EDTA Plasma (n=5)93-102%99-105%93-101%79-89%
Heparin Plasma (n=5)82-99%97-105%89-101%89-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alcohol Dehydrogenase 6 (ADH6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alcohol Dehydrogenase 6 (ADH6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alcohol Dehydrogenase 6 (ADH6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alcohol Dehydrogenase 6 (ADH6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-9283
EDTA Plasma (n=5)99-105102
Heparin Plasma (n=5)79-8879
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alcohol Dehydrogenase 6 (ADH6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alcohol Dehydrogenase 6 (ADH6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ADH6 Microplate96 Wells (12 x 8 Well Strips)
ADH6 Lyophilized Standard2 x 20 ng
100X Biotinylated ADH6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.127 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Alcohol Dehydrogenase 6 (ADH6). No significant cross-reactivity or interference between Alcohol Dehydrogenase 6 (ADH6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolADH6
Gene Full NameAlcohol dehydrogenase 6 (class V)
Alias SymbolsADH-5
NCBI Gene Id130
Protein NameAlcohol dehydrogenase 6
Description of TargetThis gene encodes class V alcohol dehydrogenase, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This gene is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' UTR, which is a steroid hormone receptor binding site. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Uniprot IDP28332
Protein Accession #NP_000663.1
Nucleotide Accession #NM_000672.3
Protein Size (# AA)368
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