Mouse Fopnl / LisH domain-containing protein FOPNL ELISA Kit (Mouse) (OKEH04768)

Catalog No: OKEH04768

Size:96T

Mouse 0610037P05Rik ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology 0610037P05Rik ELISA Kit (Mouse) (OKEH04768) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for 0610037P05Rik has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for 0610037P05Rik is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample 0610037P05Rik captured in well.
• ELISA Kit Range: 0.78-50ng/ml
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • 0610037P05Rik Microplate: 96 Wells (12 x 8 Well Strips)
  • 0610037P05Rik Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 0610037P05Rik Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.408 ng/mL
• Specificity: Natural and recombinant Mouse LisH domain-containing protein FOPNL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cep20
• Gene Full Name: centrosomal protein 20
• Alias Symbols: 0610037P05Rik;centrosomal protein 20;F;Fgfr1op N-terminal like;FGFR1OP N-terminal-like protein;Fopnl;FOP-related protein of 20 kDa;lisH domain-containing protein C16orf63 homolog;lisH domain-containing protein FOPNL.
• NCBI Gene Id: 66086
• Protein Name: Q9CZS3
• Description of Target: LisH domain-containing protein FOPNL
• Uniprot ID: https://www.uniprot.org/uniprot/Q9CZS3
• Protein Accession #: NP_079621.1
• Nucleotide Accession #: NM_025345.2
• Protein Size (# AA): 174
• Molecular Weight: 20 kDa